Journal: Royal Society Open Science

Article Title: The unexpected loss of the ‘hunger hormone’ ghrelin in true passerines: a game changer in migration physiology

doi: 10.1098/rsos.242107

Figure Lengend Snippet: Phylogenetic tree showing the loss of ghrelin in Eupasserine birds. The figure illustrates the loss of ghrelin after Acanthisitta split from Eupasserines during the Palaeogene. While the receptor (GHS-R) is present even in Eupasserines, the ligand replacing ghrelin is still unknown. The phylogenetic tree was obtained from TimeTree ( http://timetree.org/ ; last accessed 21 April 2023). The x -axis shows the age of the event in million years ago (Ma). Bird drawings were obtained from the Lynx Edicions’ Handbook of the Birds of the World with permission of Alada Gestió Empresarial S.L.

Article Snippet: The GHS-R concentration of plasma samples collected from two different bird species (the garden warbler and the common quail) was quantified using a commercially available enzyme-immunoassay kit for chicken ghrelin receptor (MyBioSource).

Techniques:

Journal: Journal of Vascular Research

Article Title: Evaluation of Metabolism-Associated Proteins in Abdominal Aortic Aneurysm

doi: 10.1159/000543768

Figure Lengend Snippet: Representative immunohistochemical detection of FNDC5 ( a , b ), follistatin ( c , d ), activin A ( e , f ), ghrelin ( g , h ), ghrelin receptor ( i , j ), and α-actin ( k ) in non-aneurysmal control aorta ( a , c , e , g , i ) and AAA ( b , d , f , h , j , k ), at ×200 magnification. Scale bar corresponds to 10 µm.

Article Snippet: Sections were incubated with primary antibodies against FNDC5 (diluted 1:200, rabbit polyclonal, #23995-I-AP, Proteintech, UK); follistatin (diluted 1:300, rabbit polyclonal, #PA5-114319, Thermo Fisher Scientific, USA); activin A (diluted 1:50, goat polyclonal, #PA5-47004, Thermo Fisher Scientific, USA); ghrelin (diluted 1:150, mouse monoclonal, #H00051738-M01, Novus Biologicals, UK); ghrelin receptor (diluted 1:150, rabbit polyclonal, #NBP2-16655, Novus Biologicals, UK); and actin α-smooth muscle (diluted 1:5,000, mouse monoclonal, #A5228, Sigma-Aldrich, USA).

Techniques: Immunohistochemical staining, Control

Journal: Journal of Vascular Research

Article Title: Evaluation of Metabolism-Associated Proteins in Abdominal Aortic Aneurysm

doi: 10.1159/000543768

Figure Lengend Snippet: Representative immunohistochemical detection of activin A ( a ), ghrelin receptor ( b ), and α-actin ( c ) in AAA, at ×400 magnification. Scale bar corresponds to 10 µm.

Article Snippet: Sections were incubated with primary antibodies against FNDC5 (diluted 1:200, rabbit polyclonal, #23995-I-AP, Proteintech, UK); follistatin (diluted 1:300, rabbit polyclonal, #PA5-114319, Thermo Fisher Scientific, USA); activin A (diluted 1:50, goat polyclonal, #PA5-47004, Thermo Fisher Scientific, USA); ghrelin (diluted 1:150, mouse monoclonal, #H00051738-M01, Novus Biologicals, UK); ghrelin receptor (diluted 1:150, rabbit polyclonal, #NBP2-16655, Novus Biologicals, UK); and actin α-smooth muscle (diluted 1:5,000, mouse monoclonal, #A5228, Sigma-Aldrich, USA).

Techniques: Immunohistochemical staining